Despite most previous studies analyzing the association of antibodies to anti-TNF response have used similar or smaller sample sizes, the number of patients used in our prospective study is relatively modest

Despite most previous studies analyzing the association of antibodies to anti-TNF response have used similar or smaller sample sizes, the number of patients used in our prospective study is relatively modest. these AZD7687 markers has not been evaluated yet. Our aim was to analyze the interaction between these four antibodies in relation to the response to anti-TNF therapy. Methods For this objective, a prospective cohort of methotrexate; conventional synthetic DMARDs; interquartile range; standard deviation Association of antibodies with anti-TNF response Multivariate linear regression was used to simultaneously test for association between anti-CCP, RF, anti-CarP and anti-PAD4 antibodies and treatment response. At the single-marker level, none of the autoantibodies were found to be significantly associated with an improvement in the DAS28 (Supplementary Table S1). We next tested for the presence of interaction effects between all six pairwise antibody combinations in relation to treatment response. We found a highly significant antibody interaction between anti-CarP and anti-PAD4 (values for association are shown for the interaction term (first column) and for the independent effect of each antibody (second and third columns). In bold, interaction P-values that are significant after correcting for multiple testing. A highly significant interaction was found for anti-CCP:RF and anti-PAD4:anti-CarP interactions with anti-TNF response. None of the remaining four antibody interactions showed a significant association, even at the nominal (P?P?=?0.044, Fig.?1). Like in the prospective cohort, the interaction was also found PJS to be positive, indicating that the simultaneous presence AZD7687 of both antibodies is associated with a better response to anti-TNF therapy. Open in a separate window Fig. 1 Validation study of the anti-CCP and RF interaction and anti-TNF response in RA. Forest plot showing the regression coefficients and 95% confidence intervals of the variables in the linear model testing the association of the two antibody combination with the response to anti-TNF therapy at week 12. Like in the prospective patient cohort, the interaction between anti-CCP and RF is statistically significant and positively associated with anti-TNF response (Beta:1.06 (0.03 to 2.10); P?