Using such antibodies or T cells as probes, many tumor antigens have been identified in various human tumors over the past 2 decades

Using such antibodies or T cells as probes, many tumor antigens have been identified in various human tumors over the past 2 decades. tumor antigens and the occurrence of immune responses to them in cancer patients. However, the relevance of such spontaneous immune responses to the clinical course has not been studied extensively. The reason for this Pyrroloquinoline quinone appears to be the difficulty of detecting anti-tumor immune responses in patients because of the generally weak antigenicity of the tumor antigens together with the lack of a reliable methodology. However, some CT antigens, including NY-ESO-1 and XAGE1 (GAGED2a), have been shown to be highly immunogenic. Besides CT antigens, the tumor suppressor gene product p53 is also known to be strongly immunogenic. A previous study on spontaneous NY-ESO-1 immune responses in cancer patients revealed that antibody responses and CD4 and CD8 T-cell responses occur concomitantly as an integrated immune response.1 Because of its sensitivity and reproducibility as well as involving a simple assay procedure, an antibody response would be a useful immune biomarker to evaluate immune responsiveness in patients. Nevertheless, it should be noted that a split immune tolerance could take place depending on the antigens.2 NY-ESO-1 antigen readily elicits a CD8 T-cell response, but p53 has been reported as a weaker elicitor, irrespective of a similar efficiency of antibody induction with these antigens. Open in a separate window Figure 1. Spontaneous immune responses TNRC23 to tumors in cancer patients. The cancer/testis (CT) antigens NY-ESO-1 and XAGE1 (GAGED2a) are strongly immunogenic, and an integrated immune response, consisting of an antibody response and CD4 and CD8 T-cell responses is frequently elicited spontaneously. The antibody response is a useful biomarker of immune responses because of its sensitivity and reproducibility, as well as involving a simple assay procedure. CD4 T-cell responses to CT antigens would be enhanced by the antigen-presenting cells (APC) that efficiently internalize the antigen/antibody complex via the Fc receptor (FcR) and promote the antibody response via IL-4 and IL-5 cytokines, and the CD8 T-cell response via IL-2 and IFN. CD8 T cells Pyrroloquinoline quinone lyse tumor cells via Fas-FasL, perforin, and/or granzyme. Tregs and MDSCs suppress CD4 and CD8 T cells. By the detection of antibody Pyrroloquinoline quinone responses against CT antigens, we have investigated the clinical relevance of such spontaneous immune responses in cancer patients. In gastric cancer patients, we previously showed that an NY-ESO-1 antibody response was present at 3C4% in stage I and II, Pyrroloquinoline quinone and 20C25% in stage III and IV patients, suggesting a higher antibody response rate in more advanced stage patients.3 Analysis of the overall survival in 310 gastric cancer patients with stages I to IV showed no difference between antibody-positive and -negative patients. However, in the 126 patients in stages III and IV, the overall survival of antibody positive patients was prolonged, although not significantly. It should be noted that NY-ESO-1 expression itself is tumorigenic, and patients with NY-ESO-1-antigen-positive tumors showed a shorter survival compared to patients with antigen-negative tumors in various cancer types.4 In our recent prospective study on advanced stage (stages IIIB and IV) lung adenocarcinoma patients, patients with antibody responses to XAGE1 (GEGED2a) showed a significantly prolonged overall survival compared to patients with no antibody responses.5 XAGE1 (GAGED2a) antigen expression was a worse predictor in patients with EGFR-mutated tumors. The XAGE1 (GAGED2a) antibody frequency in the advanced lung adenocarcinoma patients was similar (approximately 20%) to the frequency of NY-ESO-1 antibody responses in advanced gastric cancer patients. However, the patient cohort is more restricted at advanced stages in lung cancer compared to gastric cancer patients. Daudi, et?al.6 recently reported that ovarian cancer patients with antibody responses to any of the MAGE family antigens showed a shorter survival compared to patients without antibody responses. In their study, patients with all stages were included, and so the shorter survival of advanced cancer patients should be carefully considered, as they discussed. A stage-controlled study is necessary to elucidate the effect of the antibody response on the clinical course. A similar overall survival-shortening effect of the antibody response was observed in patients with antibody against p53.7 Caution, as described in Pyrroloquinoline quinone Daudi’s report, should also be exercised when interpreting the results for p53. Thus, in order to evaluate the clinical significance of the spontaneous antibody response in cancer patients, the clinical relevance of antigen expression should be carefully examined. The CT antigen expression itself generally worsens survival..